Tesco has abandoned 24-hour trading at 76 of its larger stores as part of plans to further streamline its business.
The multiple blamed the growth in online shopping for keeping customers away from its stores at unsociable hours. The figure represents around one in five of its 24-hour stores.
The stores will now close at midnight and reopen at 6am, with the changes taking place between March 14 and April 11.
A spokesperson for the company said: "The move to reduce hours means more Tesco colleague time will be freed up to replenish products on the shelves overnight, which will mean better availability of products and better looking stores when they open their doors to customers each morning."
The supermarket chain, which began opening its stores for 24 hours in 1996, has seen its profits slide thanks to increased competition from discount retailers such as Aldi and Lidl.
To find out which stores near you will close overnight, use our interactive map.
It will also close its two London-based food to go stores, which were designed to take on sandwich shops, on March 4.
The stores in Philpot Lane, near Monument, and Villiers Lane, close to Charing Cross train station, failed to attract enough shoppers and were deemed unprofitable because of high rents.